Lion's Mane tincture is from pure Lions Mane Mushroom grown here in the USA. It is also mixed with pure alcohol. Lions Mane has health benefits that are so many it is hard to list all that it is known to do. The benefits are extensive and cover the brain, nervous system, immune system, and heart. It is found to diminish dementia, diabetes, insomnia, heart issues and so much more. USAGE: 1 dropper twice a day. Approximately 1 month supply per bottle. This is a 3:5 ratio.